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Cloning of the cDNA and localization of the gene encoding human NRBP, a ubiquitously expressed, multidomain putative

J D Hooper1, E Baker, S M Ogbourne

  • 1Cellular Oncology Laboratory, University of Queensland, Brisbane, Queensland, 4029, Australia.

Genomics
|June 14, 2000
PubMed

Insights

Researchers identified NRBP, a novel adapter protein with domains for nuclear receptors and SH2 proteins. This protein, found ubiquitously in human tissues and tumors, may link key cellular signaling pathways.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Genetics

Background:

  • Adapter proteins are crucial for assembling signaling complexes, regulating cellular pathways.
  • Understanding novel adapter proteins can elucidate complex biological processes.

Purpose of the Study:

  • To identify and characterize a novel human adapter protein, NRBP.
  • To investigate the structural features, expression, and potential function of NRBP.

Main Methods:

  • Human cDNA library screening to identify NRBP.
  • Bioinformatic analysis of NRBP protein domains and gene localization.
  • mRNA expression analysis in normal tissues and tumor cell lines.
  • In vitro translation assays.

Main Results:

  • Identified a novel human cDNA encoding NRBP, a multidomain adapter protein.
  • NRBP possesses nuclear receptor binding motifs, an SH2 domain binding site, a kinase-like domain, and PEST sequences.
  • NRBP mRNA is ubiquitously expressed in normal human tissues and tumor cell lines.
  • The NRBP gene is located on human chromosome 2p23.
  • In vitro translation produced multiple NRBP protein isoforms.

Conclusions:

  • NRBP is a novel multidomain adapter protein with predicted roles in linking nuclear receptor and SH2-mediated signaling pathways.
  • Ubiquitous expression suggests a fundamental role for NRBP in normal physiology and potentially in tumorigenesis.

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