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CD8+ cells suppress oil-induced arthritis
A M Jansson1, J C Lorentzen, A Bucht
1Department of Medicine, Unit of Rheumatology, Karolinska Institute, Stockholm, Sweden. asa.jansson@cmm.ki.se
Clinical and Experimental Immunology
|June 9, 2000
Summary
CD8+ T cells play a crucial role in regulating oil-induced arthritis in rats. Depleting these cells accelerated disease onset, indicating their protective function in this specific type of arthritis.
Area of Science:
- Immunology
- Autoimmune Diseases
- Rat Models
Background:
- Oil-induced arthritis is a polyarthritis model in DA rats.
- The role of specific immune cell populations in disease development is not fully understood.
Purpose of the Study:
- To investigate the role of CD8+ T cells, gammadelta T cells, NK cells, and NK T cells in adjuvant-induced arthritis.
- To determine the contribution of these cells to disease susceptibility and resistance.
Main Methods:
- Flow cytometry analysis of inguinal lymph nodes in DA and LEW.1AV1 rats.
- In vivo depletion of CD8+ T cells and gammadelta T cells using monoclonal antibodies (MoAbs).
- Monitoring of arthritis development after adjuvant injection.
Main Results:
- NK T cells may be protective, but anti-NKR-P1 antibody treatment had inconsistent effects.
- CD8+ T cell proportions did not differ between strains, but their depletion accelerated disease onset in DA rats.
- Gammadelta T cell depletion did not affect disease course, suggesting CD8+ T cells are alphabeta T cells or NK cells.
Conclusions:
- CD8+ T cells are critical regulators of adjuvant-induced arthritis development.
- The findings highlight the importance of CD8+ T cells in controlling this specific polyarthritis model.