Related Experiment Video

Updated: Aug 10, 2026

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia
09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

Activating protein-1, nuclear factor-kappaB, and serum response factor as novel target molecules of the

S K Lee1, S Y Na, S Y Jung

  • 1Center for Ligand and Transcription, Department of Biology, Chonnam National University, Kwangju, Korea.

Molecular Endocrinology (Baltimore, Md.)
|June 10, 2000
PubMed

Insights

The cancer-associated coactivator ASC-2 interacts with transcription factors serum response factor (SRF), activating protein-1 (AP-1), and nuclear factor-kappaB (NFkappaB). ASC-2 enhances their activity and may play a role in tumorigenesis.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Regulation

Background:

  • ASC-2 (Amplifier of the Splicing Complex 2) is a known coactivator for nuclear receptors involved in cancer.
  • It interacts with other coactivators like SRC-1 and CBP/p300.
  • The full range of ASC-2's interactions and functions in gene regulation is not completely understood.

Purpose of the Study:

  • To identify novel target molecules of the coactivator ASC-2.
  • To investigate the interaction of ASC-2 with specific transcription factors.
  • To determine the functional consequences of ASC-2's interactions on gene transactivation and its role in tumorigenesis.

Main Methods:

  • Yeast two-hybrid screening to identify ASC-2 interacting proteins.
  • Glutathione S-transferase (GST) pull-down assays to confirm interactions.
  • Mammalian cell cotransfection assays to assess effects on gene transactivation.

Main Results:

  • ASC-2 directly interacts with serum response factor (SRF), c-Jun/c-Fos (AP-1), and p50/p65 (NFkappaB).
  • ASC-2 potentiates the transactivation activity of SRF, AP-1, and NFkappaB in a dose-dependent manner.
  • ASC-2 alleviates transrepression between nuclear receptors and AP-1 or NFkappaB.

Conclusions:

  • ASC-2 functions not only as a nuclear receptor coactivator but also mediates transactivation by SRF, AP-1, and NFkappaB.
  • These newly identified interactions suggest a broader role for ASC-2 in gene regulation.
  • ASC-2's involvement in the activation of these transcription factors may contribute to cancer development.

More Related Videos

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...