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Related Experiment Videos

Activated clotting time (ACT) testing: analysis of reproducibility.

M L Zucker1, C Jobes, M Siegel

  • 1International Technidyne Corporation, Edison, NJ 08820, USA.

The Journal of Extra-Corporeal Technology
|June 10, 2000
PubMed
Summary

Activated Clotting Time (ACT) tests demonstrate precision well below the 10% CV standard. This study confirms Hemochron ACT test tubes offer reliable heparin monitoring in cardiac surgery, using both lab and clinical data.

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Area of Science:

  • Cardiovascular Surgery
  • Hematology
  • Medical Device Evaluation

Background:

  • Activated Clotting Time (ACT) is the standard for heparin monitoring in cardiac surgery.
  • Current precision standards (10% CV) may not reflect modern test performance.
  • Recent precision data for ACT testing is limited.

Purpose of the Study:

  • To evaluate the precision of Hemochron FTCA510 (celite) and KACT (kaolin) ACT test tubes.
  • To determine if ACT test variability meets or exceeds the 10% CV standard.
  • To assess ACT test reliability in laboratory and clinical settings.

Main Methods:

  • Retrospective analysis of laboratory and clinical data for Hemochron ACT tests.
  • Evaluation of reproducibility using multiple lots of control plasma and fresh heparinized blood.

Related Experiment Videos

  • Analysis of coefficient of variation (CV) across various heparin concentrations and clinical samples.

    • Main Results:

    • Both celite and kaolin ACT tests showed average CVs significantly below 10% in laboratory studies (e.g., celite 5.4% normal, 4.0% abnormal; kaolin 4.5% normal, 3.8% abnormal).
    • Precision was even higher with fresh whole blood compared to control plasma.
    • Clinical evaluation demonstrated minimal differences in paired ACT values (average 1.8% difference).

    Conclusions:

    • Hemochron ACT test tubes (celite and kaolin) exhibit precision significantly better than the 10% CV standard.
    • These ACT tests provide reliable heparin anticoagulation monitoring for cardiac surgery.
    • The study validates the accuracy and consistency of Hemochron ACT testing in real-world clinical use.