Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The nucleus: a highly organized but dynamic structure.

P Gonzalez-Melendi1, A Beven, K Boudonck

  • 1John Innes Centre, Colney, Norwich NR4 7UH, U.K.

Journal of Microscopy
|June 10, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rising Tuberculosis in Ireland: A Renewed Challenge in a Changing World.

Irish medical journal·2026
Same author

Defining the Clinical Target Volume in Postoperative Thymic Epithelial Tumours - A Clinical Practice Guide for UK Clinical Oncologists.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

CODAK: Real-World Clinical Outcomes of Patients With Unresectable, Stage III Non-Small Cell Lung Cancer Treated With Durvalumab After Chemoradiotherapy in the United Kingdom.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Congenital tuberculosis following disseminated TB in pregnancy: a case report.

QJM : monthly journal of the Association of Physicians·2025
Same author

'I can't relate to it': Exploring autistic and non-autistic adults' perceptions of the Australian Physical Activity and Sedentary Behaviour Guidelines.

Health promotion journal of Australia : official journal of Australian Association of Health Promotion Professionals·2024
Same author

Zoonotic tuberculosis and pathogen whole genome sequencing.

Irish medical journal·2024
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Plant cell nuclei are highly organized, with transcription sites found throughout. Despite regular chromosome arrangement, transcription is not polarized. Coiled bodies are dynamic structures within the nucleus.

Area of Science:

  • Cell Biology
  • Plant Science
  • Genetics

Background:

  • The plant nucleus is a complex organelle with distinct subregions like the nucleolus, interphase chromosome territories, and coiled bodies.
  • Understanding the spatial organization and dynamics of transcription within the nucleus is crucial for gene regulation.

Purpose of the Study:

  • To visualize and characterize transcription sites in plant cells using BrUTP incorporation.
  • To investigate the relationship between nuclear organization, chromosome territories, and transcription site localization.
  • To explore the dynamic behavior of nuclear suborganelles, such as coiled bodies.

Main Methods:

  • Visualization of transcription sites at light and electron microscopy levels through BrUTP incorporation.
  • Analysis of interphase chromosome territory organization in wheat using microscopy.

Related Experiment Videos

  • Tracking the movement of coiled bodies using green fluorescent protein-tagged spliceosomal protein U2B".

    • Main Results:

    • Transcription sites were observed as dispersed foci within the nucleolus and nucleoplasm.
    • Plant interphase chromosome territories exhibit a regular Rabl configuration with clustered centromeres and telomeres at the nuclear periphery.
    • No polarization of transcription sites was observed relative to chromosome territories, despite the nucleus's polarized structure.
    • Coiled bodies were shown to be dynamic, moving and coalescing, suggesting a role in nuclear transport.

    Conclusions:

    • Transcription in plant cells occurs at numerous dispersed sites, including within the nucleolus.
    • The regular, polarized structure of plant nuclei does not dictate a polarized distribution of transcription.
    • Coiled bodies are mobile nuclear structures potentially involved in transport processes within the nucleus and nucleolus.