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Updated: Jul 11, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
alpha-Amylase production by free and immobilized Bacillus subtilis
E Duran-Paramo1, O Garcia-Kirchner, J F Hervagault
1Laboratoire de Technologie Enzymatique (UPRES-A CNRS 6022), Université de Technologie de Compiègne, France.
Abstract:
The effect of glucose on the alpha-amylase production by Bacillus subtilis ATCC-21556 was studied. Initial glucose concentrations up to 20 g/L were found to be directly proportional to the specific alpha-amylase production in an immobilized-cell batch system, whereas a free-cell batch system presented an inversely proportional relationship with the initial glucose concentration. This might be owing to the alpha-amylase repression by the glucose present in the culture medium. Three hundred eighty-five percent of the specific alpha-amylase production with the free-cell system was produced by the immobilized-cell batch culture.
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