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Published on: December 23, 2016
Enhancement of selectivity for producing gamma-cyclodextrin.
G Matioli1, G M Zanin, F F de Moraes
1Pharmacy and Pharmacology Department, State University of Maringá, PR, Brazil.
This study optimized cyclodextrin (CD) production using Bacillus firmus cyclodextrin-glycosyl-transferase (CGTase). Cornstarch and glycyrrhizin significantly enhanced gamma-cyclodextrin yield, demonstrating an effective strategy for its targeted production.
Area of Science:
- Enzymology
- Carbohydrate Chemistry
- Biotechnology
Background:
- Cyclodextrins (CDs) are cyclic oligosaccharides with diverse applications.
- Cyclodextrin-glycosyl-transferase (CGTase) is crucial for CD biosynthesis.
- Optimizing CGTase activity and substrate specificity is key for efficient CD production.
Purpose of the Study:
- To investigate the impact of different starch sources on CD yield and gamma-CD selectivity using CGTase from Bacillus firmus.
- To evaluate the effect of glycyrrhizin as a complexing agent on gamma-CD production.
Main Methods:
- Enzymatic conversion using CGTase from Bacillus firmus.
- Testing various starch substrates (maltodextrin, rice, potato, cassava, corn starch) hydrolyzed to D.E. 10.
- Reaction conditions: 50°C, pH 8.0, 24h incubation.
- Addition of glycyrrhizin (2.5% w/v) to the reaction mixture.
Main Results:
- Cornstarch was identified as the optimal substrate for gamma-CD production.
- Glycyrrhizin addition increased gamma-CD yield approximately fourfold compared to maltodextrin.
- Total CD yield remained largely unchanged despite increased gamma-CD selectivity.
Conclusions:
- Bacillus firmus CGTase demonstrates potential for high gamma-CD yield.
- Cornstarch as a substrate and glycyrrhizin as a complexant significantly enhance gamma-CD production efficiency.
- This method offers a promising approach for targeted gamma-CD synthesis.
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