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Updated: Aug 19, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
Published on: October 20, 2014
Sequential steps in clathrin-mediated synaptic vesicle endocytosis
L Brodin1, P Löw, O Shupliakov
1Department of Neuroscience, The Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, S-171 77, Sweden. lennart.brodin@neuro.ki.se
Abstract:
Synaptic vesicles are recycled with remarkable speed and precision in nerve terminals. A major recycling pathway involves clathrin-mediated endocytosis at endocytic zones located around sites of release. Different 'accessory' proteins linked to this pathway have been shown to alter the shape and composition of lipid membranes, to modify membrane-coat protein interactions, and to influence actin polymerization. These include the GTPase dynamin, the lysophosphatidic acid acyl transferase endophilin, and the phosphoinositide phosphatase synaptojanin. Protein perturbation studies in living nerve terminals are now beginning to link the actions of these proteins with morphologically defined steps of endocytosis.
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