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TNF gene polymorphism and its relation to intracerebral production of TNFalpha and TNFbeta in AD
E Tarkowski1, A M Liljeroth, A Nilsson
1Department of Rheumatology, University of Göteborg, Sweden.
Objective:
To analyze the extent of tumor necrosis factor-alpha (TNFalpha) and TNFbeta gene polymorphism in patients with AD and to relate it to intrathecal levels of these cytokines.
Methods:
Analyses of TNFalpha and TNFbeta gene polymorphism were performed using PCR in 52 patients with AD and in 25 control subjects, and the levels of corresponding cytokines were analyzed using ELISA.
Results:
Patients with AD displayed significantly higher intrathecal levels of TNFalpha, but not TNFbeta, compared with the control subjects. The levels of these cytokines did not differ significantly in patients displaying different alleles of the TNF gene.
Conclusions:
Results indicate that increased intrathecal production of TNFalpha in AD is preferentially controlled by environmental stimuli rather than genetic makeup.