Effect of anti-inflammatory agent on L-glutamine-D-fructose-6-phosphate transaminase

The enzyme L-glutamineD-fructose-6-phosphate aminotransferase (EC 2.6.1.16) was isolated, partially characterized, and purified from the gastric mucosa of dogs. A new method, using 14C-fructose-6-phosphate, has been developed for the estimation of the enzyme activity. Several classes of standard anti-inflammatory agents including acetyl-salicylic acid, salicylic acid, flufenamic acid, phenyl-butazone, indometacin, mefenamic acid, oxyphenyl-butazone, antipyrine, aminophenazone, allopurinol, cortisol, and dimethylsulfoxide (DMSO) were found to be inhibitors of this enzyme. The property of inhibition of this amino-transferase could, thus, be used as a rapid in vitro test method for the primary screening of potential anti-inflammatory agents.