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[Bacterial endotoxinemia in children with intestinal dysbacteriosis]
E A Lykova1, V M Bondarenko, A A Vorob'ev
1Gamaleya Research Institute of Epidemiology and Microbiology, Sechenov Medical Academy, Moscow, Russia.
Insights
Children with intestinal dysbiosis often have endotoxin in their plasma. A probiotic containing Bifidumbacterin forte reduced endotoxin levels, suggesting a benefit for gastrointestinal health.
Area of Science:
- Pediatric Gastroenterology
- Microbiology
- Immunology
Background:
- Intestinal dysbiosis is common in children with gastrointestinal diseases.
- Endotoxemia, indicated by plasma endotoxin, is frequently observed in these patients.
- Secretory immunoglobulin A (sIgA) plays a role in intestinal immunity.
Purpose of the Study:
- To investigate the prevalence of endotoxemia in children with gastrointestinal diseases.
- To explore the relationship between endotoxemia, intestinal microflora, and sIgA levels.
- To evaluate the efficacy of a probiotic containing Bifidumbacterin forte in managing endotoxemia.
Main Methods:
- Fecal bacterial analysis to assess intestinal microflora.
- Radial immunodiffusion to measure fecal secretory immunoglobulin A (sIgA).
- Limulus (LAL) test for plasma endotoxin detection.
- Analysis of probiotic Bifidumbacterin forte's impact on endotoxinemia.
Main Results:
- Endotoxin was detected in the plasma of 71.1% of children with intestinal dysbiosis.
- Endotoxemia frequency correlated with dysbiosis severity and sIgA levels.
- Inclusion of Bifidumbacterin forte in therapy reduced endotoxinemia detection rates.
- Probiotic use showed a trend towards normalizing intestinal microflora.
Conclusions:
- Endotoxemia is a significant finding in pediatric gastrointestinal diseases associated with dysbiosis.
- Fecal sIgA levels and dysbiosis severity are linked to endotoxemia.
- Bifidumbacterin forte may be a valuable adjunct therapy for reducing endotoxemia and improving gut health in children.
Abstract:
34 children with gastrointestinal diseases of infectious, allergic and mixed etiology were examined. The state of normal microflora in the large intestine as indicated by fecal bacterial charts and the level of secretory immunoglobulin A (sIgA) in the contents of the intestine as indicated by the results of radial immunodiffusion were studied. In addition, the content of endotoxin in the children's plasma was determined with the use of the Limulus (LAL) test. The presence of endotoxin in the plasma of children with intestinal dysbiosis was determined in 71.1% of cases. The frequency of the detection of antigenemia was found to be related to the severity of manifestations of dysbiotic changes in the intestine and to the level of sIgA in fecal supernatants. The inclusion of the probiotic preparation Bifidumbacterin forte containing live bifidobacteria adsorbed on activated charcoal into the complex therapy of digestive tract diseases ensured a decrease in the detection rate of endotoxinemia, which correlated with the tendency towards the normalization of defective intestinal microflora.