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Related Experiment Videos

Mast cell basic fibroblast growth factor in silicosis.

H Hamada1, V Vallyathan, C D Cool

  • 1Division of Environmental and Occupational Health Sciences, Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USA.

American Journal of Respiratory and Critical Care Medicine
|June 14, 2000
PubMed
Summary

Mast cells (MC) producing basic fibroblast growth factor (bFGF) are elevated in silicotic lungs, correlating with increased collagen. These MC may drive fibrogenesis in silicosis.

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Area of Science:

  • Pulmonary Pathology
  • Cell Biology
  • Fibrosis Research

Background:

  • Silicosis is a progressive fibrotic lung disease caused by silica dust inhalation.
  • Mast cells (MC) and fibrogenic growth factors are implicated in fibrotic diseases.
  • The specific role of MC and basic fibroblast growth factor (bFGF) in silicosis pathogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the involvement of mast cells (MC) and their fibrogenic growth factors, specifically basic fibroblast growth factor (bFGF), in the development of silicosis.
  • To quantify MC and bFGF in lung tissue from silicotic and control subjects.
  • To correlate the presence and distribution of MC and bFGF with fibrotic changes in silicotic lung tissue.

Main Methods:

  • Quantitative immunohistochemistry was employed to detect MC tryptase and bFGF in lung tissue samples.

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  • Morphometric analysis was used to determine the volume density (V(v)) of MC and bFGF-positive (bFGF(+)) cells.
  • Correlation analyses were performed to assess the relationship between MC, bFGF, collagen/reticulin fibers, and cell densities within silicotic nodules.

    • Main Results:

    • The volume density of MC and bFGF-positive cells was significantly increased in silicotic lungs compared to controls.
    • Most MC in silicotic lungs contained bFGF, showing a strong positive correlation.
    • Increased collagen/reticulin fiber density in silicosis correlated positively with bFGF-positive cells, but negatively with MC and other cell densities in mature nodules.

    Conclusions:

    • Mast cells producing bFGF are significantly elevated in silicotic lung tissue.
    • The presence and distribution of bFGF-positive MC correlate with fibrotic changes, particularly collagen deposition.
    • MC producing bFGF are suggested to play a crucial role in the fibrogenesis characteristic of silicosis.