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A simple, solid-phase binding assay for the nuclear import receptor karyopherin alpha. Part 2: competitive binding
S B Park1, B M Reedy, R F Standaert
1Department of Chemistry, Texas A&M University, College Station 77842-3012, USA.
Bioorganic & Medicinal Chemistry Letters
|June 15, 2000
Abstract:
A qualitative assay for the evaluation of soluble ligands of the nuclear import receptor karyopherin alpha has been developed. The assay relies on competition with an immobilized ligand, the nuclear localization signal (NLS) from nucleoplasmin, for binding to the receptor, which is detected by an enzyme-linked colorimetric method.