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Echovirus 5: infectious transcripts and complete nucleotide sequence from uncloned cDNA.
A M Lindberg1, S Johansson, A Andersson
1Institute of Natural Sciences, University of Kalmar, Sweden. michael.lindberg@ng.hik.se
Virus Research
|June 15, 2000
Summary
Echovirus 5 (EV5) is closely related to coxsackievirus B viruses, based on its complete genome sequence. This finding helps understand EV5
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Echovirus 5 (EV5) is associated with neurological and exanthematic diseases.
- Understanding EV5's genetic relationship to other enteroviruses is crucial for disease research and cellular interaction studies.
Purpose of the Study:
- To determine the complete nucleotide sequence of Echovirus 5 (EV5).
- To elucidate the phylogenetic relationship of EV5 to other enteroviruses.
- To investigate EV5's potential interactions with host cells.
Main Methods:
- Complete EV5 genome amplification using reverse transcription-polymerase chain reaction (RT-PCR).
- High-throughput sequencing of amplified EV5 genome fragments.
- Long-distance PCR for amplifying full-length EV5 genomes.
- In vitro transcription and transfection of EV5 RNA into cell cultures.
Main Results:
- The complete nucleotide sequence of EV5 was determined, revealing a typical enterovirus genome organization.
- EV5 was confirmed to be infectious, with transfected RNA leading to viral replication.
- Sequence analysis classified EV5 as a coxsackievirus B-like enterovirus.
- Variations between EV5 and coxsackievirus B3 (CVB3) were primarily on exposed capsid residues.
Conclusions:
- EV5 shares significant genetic and structural similarities with coxsackievirus B viruses.
- The observed sequence variations suggest recent divergence from group B coxsackieviruses.
- This study provides a foundation for further research into EV5 pathogenesis and host-cell interactions.