Renal test dyes III. Effect of dyes suitable for renal passage time measurements on renal function

The effects of the triaryl-methane dyes Lissamine Green SF (LF SF). Lissamine Green BN (LG BN), Lissamine Green V (LG V) and Kiton Pure Blue V (KPB V) on renal functions of rats and on sodium transport across isolated frog skin have been investigated. The experiments failed to show any natriuretic or diuretic effects of the purified dyes on the rat kidney. In the continuous infusion experiments, which lasted 3 1/2 h, the amount of the dye infused was equivalent to a passage time measurement every 3 min. In rapid injection experiments LG V was injected every 5 min for 1 h. The dyes did not have any effect on insulin clearance either. Previously reported effects of the dyes can be partly explained by the presence of sodium sulfate in commercially available dyes. Experiments with LG V showed that the dye also has no effect on the potential difference or short circuit current across the isolated frog skin. This supports their usefulness as renal test dyes.