Related Experiment Video
Updated: Jul 15, 2026
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
Published on: September 16, 2011
A new method for histamine release from purified peripheral blood basophils using monoclonal antibody-coated magnetic
H Nishi1, S Nishimura, M Higashiura
1Diagnostic Science Division, Shionogi and Co., Ltd., 2-5-1 Mishima, Settsu-shi, Osaka, Japan. hiroshi.nishi@shionogi.co.jp
Abstract:
A new method for evaluating histamine release from purified basophils was developed. Basophil-containing leukocytes were directly purified from a small amount of peripheral blood using monoclonal antibody BA312-coated magnetic beads. The purified basophils still rosetted to magnetic beads maintained a normal response to anti-IgE and to dust mite allergen in comparison with the conventional method using washed leukocytes. This methodology facilitates the purification of basophils, anti-IgE- and allergen-induced histamine release, and subsequent histamine determination within only 3 h. The released histamine was analyzed by an enzyme-linked immunosorbent assay (ELISA) with a characteristic detection profile. Since all steps were performed in 96-well microplates, many clinical samples could be analyzed at the same time, permitting easy applications in routine laboratories.
