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Related Experiment Videos

Modification of tight junction function by protein kinase C isoforms.

H Clarke1, C W Marano, A Peralta Soler

  • 1Lankenau Medical Research Center, Wynnewood, PA 19096, USA.

Advanced Drug Delivery Reviews
|June 16, 2000
PubMed
Summary

Protein kinase C activation affects tight junction permeability, potentially impacting epithelial barriers and cancer development. This pathway

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Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Tight junctions regulate paracellular permeability in epithelial tissues.
  • Signal transduction pathways, particularly protein kinase C (PKC), influence tight junction function.
  • Dysregulation of tight junctions is implicated in various diseases, including cancer.

Purpose of the Study:

  • To summarize the regulation of tight junction permeability by signal transduction pathways.
  • To emphasize the role of protein kinase C isoforms in regulating paracellular permeability.
  • To explore the implications of PKC activation in epithelial barrier function, carcinogenesis, and potential diagnostic markers.

Main Methods:

  • Review of studies utilizing phorbol ester activators of protein kinase C.

Related Experiment Videos

  • Analysis of the effects of PKC activation on epithelial barrier function and solute permeability.
  • Examination of PKC-mediated phosphorylation of tight junctional proteins and downstream signaling.

    • Main Results:

    • Protein kinase C activation significantly increases paracellular permeability to a wide range of solutes.
    • PKC activation may facilitate growth factor leakage, contributing to epithelial carcinogenesis.
    • PKC's effects on tight junction permeability are likely mediated by downstream kinases and phosphatases.
    • The role of PKC in transepithelial drug delivery is questionable.

    Conclusions:

    • Protein kinase C plays a critical role in modulating tight junction permeability.
    • PKC-induced tight junction leakiness is associated with epithelial carcinogenesis.
    • Tight junction leakiness in transformed epithelia may serve as a potential early cancer diagnostic marker.