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Binding of IgM by human T lymphocytes.
Scandinavian Journal of Immunology
|January 1, 1976
Summary
Human lymphocytes form rosettes with antibody-coated ox erythrocytes, specifically via T-lymphocytes. This IgM-mediated binding, observed in blood, tonsils, and thymus, offers insights into T-cell interactions.
Area of Science:
- Immunology
- Cell Biology
Background:
- Rabbit IgM antibodies can sensitize ox erythrocytes.
- Lymphocyte interactions with antibody-coated cells are crucial in immune responses.
Purpose of the Study:
- To investigate the interaction between antibody-sensitized erythrocytes and human blood cells.
- To identify the specific lymphocyte subset involved in rosette formation.
Main Methods:
- Rosette formation assays using IgM-sensitized ox erythrocytes and human lymphocytes.
- Inhibition studies using human IgM and its Fc fragments.
- Immunological marker tests and cell separation techniques.
Main Results:
- Human blood lymphocytes form rosettes with IgM-sensitized ox erythrocytes.
- Granulocytes, monocytes, and macrophages do not form rosettes.
- Rosette formation is inhibited by human IgM and its Fc fragments.
- The rosetting lymphocytes were identified as T-lymphocytes.
Conclusions:
- A subpopulation of human T-lymphocytes binds to IgM antibodies via Fc receptors.
- This IgM-binding T-lymphocyte population is present in peripheral blood, tonsils, and thymus.
- The findings contribute to understanding T-lymphocyte heterogeneity and function.