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Characterization of dextran-sulfate-sensitive cells.

E Gronowicz, P Biberfeld, B Wahren

    Scandinavian Journal of Immunology
    |January 1, 1976
    PubMed
    Summary
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    Dextran-sulfate (DS) primarily activates lymphocytes in the spleen. In bone marrow, DS may indirectly activate various hematopoietic cells, but it is not a direct stem cell mitogen.

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • Polyclonal B-cell activators (PBA) like dextran-sulfate (DS) are crucial tools for studying immune cell responses.
    • Understanding which specific cell types respond to mitogens is essential for deciphering immune system function.

    Purpose of the Study:

    • To identify the specific cell types undergoing mitotic responses to dextran-sulfate (DS) in murine fetal liver, bone marrow, and spleen.
    • To investigate the mechanism of DS-induced cellular activation, particularly in bone marrow hematopoietic stem cells.

    Main Methods:

    • Morphological analysis of cells from murine fetal liver, bone marrow, and spleen after stimulation with DS.
    • Assessment of DNA synthesis and cell proliferation in response to DS.
    • Evaluation of DS's effect on hematopoietic stem cell colony growth in vitro.

    Related Experiment Videos

  • Analysis of phagocytic cell populations in DS-stimulated cultures.

    • Main Results:

    • In spleen, DS predominantly activated lymphocytes morphologically resembling lymphoblasts.
    • Bone marrow cultures showed mitotic activity in various hematopoietic cells, including lymphoblasts, monocytes, megakaryocytes, and myeloblasts, though differentiation from spontaneous proliferation was difficult.
    • DS increased phagocytic cells in bone marrow and spleen cultures, but adherent cells were not essential for DS-induced DNA synthesis in spleen.
    • DS did not directly promote hematopoietic stem cell colony growth but supernatants from DS-activated cultures did, suggesting indirect activation of non-lymphoid stem cells.

    Conclusions:

    • The primary cell population activated by DS in the spleen is lymphocytes.
    • In bone marrow, DS appears to indirectly activate various cell types, rather than directly interacting with them as a stem cell mitogen.
    • DS may play a role in the indirect activation of non-lymphoid stem cells through factors released by activated spleen and bone marrow cells.