Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The platelet-endothelial cell--VIII axis.

R L Nachman, E A Jaffe

    Thrombosis and Haemostasis
    |February 29, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Human endothelial cells produce von Willebrand factor, a protein essential for platelet function. Platelets store this factor internally and on their surface, acting as a significant reservoir.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    High prevalence of adrenal insufficiency in patients with sickle cell disease: results from a community hospital in the U.S.

    Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2013
    Same author

    Gastric marginal zone B cell lymphoma of the duodenum.

    Case reports in gastroenterology·2011
    Same author

    The Jeremiah Metzger Lecture. Hypercoagulable states: challenges and opportunities.

    Transactions of the American Clinical and Climatological Association·2001
    Same author

    The case for more U.S. medical students.

    The New England journal of medicine·2001
    Same author

    Tumor necrosis factor employs a protein-tyrosine phosphatase to inhibit activation of KDR and vascular endothelial cell growth factor-induced endothelial cell proliferation.

    The Journal of biological chemistry·2001
    Same author

    Histidine-rich glycoprotein inhibits the antiangiogenic effect of thrombospondin-1.

    The Journal of clinical investigation·2001
    Same journal

    Discontinuation of Oral Anticoagulation After Successful Catheter Ablation for Atrial Fibrillation: Meta-analysis of Randomized Clinical Trials.

    Thrombosis and haemostasis·2026
    Same journal

    Novel/Recurrent Variants in Pakistani Glanzmann Thrombasthenia and Glanzmann-like bleeding diathesis: Insights from NGS Analysis.

    Thrombosis and haemostasis·2026
    Same journal

    Anti-TFPI Single-Domain Antibodies: Novel Rebalancing Therapies for Hemophilia and Other Rare Bleeding Disorders.

    Thrombosis and haemostasis·2026
    Same journal

    The Interaction of Fibrin with Endothelial Cell Receptor N-Cadherin Promotes Fibrin-Dependent Angiogenesis.

    Thrombosis and haemostasis·2026
    Same journal

    Antiphospholipid Antibodies and Atrial Fibrillation: Clinical Implications and the TaPL AF Study Design.

    Thrombosis and haemostasis·2026
    Same journal

    Prasugrel vs. Ticagrelor: Can TUXEDO-2 Settle the Debate?

    Thrombosis and haemostasis·2026
    See all related articles

    Area of Science:

    • Hematology
    • Cell Biology
    • Vascular Biology

    Background:

    • Endothelial cells are known to synthesize and secrete various proteins involved in hemostasis.
    • Platelets play a crucial role in blood clotting and vascular integrity.
    • Factor VIII antigen and von Willebrand factor (vWF) are critical for normal hemostasis.

    Purpose of the Study:

    • To investigate the synthesis and secretion of factor VIII antigen and von Willebrand factor by cultured human endothelial cells.
    • To determine the presence and localization of factor VIII antigen and von Willebrand factor activity within human platelets.
    • To assess the contribution of platelets to the total pool of von Willebrand factor.

    Main Methods:

    • Culturing human endothelial cells and analyzing their secreted products.

    Related Experiment Videos

  • Fractionation of human platelets into subcellular components (membrane and granule fractions).
  • Assaying fractions for factor VIII antigen and von Willebrand factor activity.

    • Main Results:

    • Cultured human endothelial cells were confirmed to synthesize and secrete proteins with factor VIII antigen and von Willebrand factor activity.
    • Subcellular fractions of human platelets (membrane and granule) also demonstrated the presence of factor VIII antigen and von Willebrand factor activity.
    • Circulating platelets were identified as a significant reservoir, containing approximately 15% of the factor VIII antigen found in platelet-poor plasma.

    Conclusions:

    • Endothelial cells are the source of von Willebrand factor, which is subsequently stored in platelets.
    • Normal platelets contain both surface-bound and intracellularly stored von Willebrand factor.
    • Von Willebrand factor, synthesized by endothelial cells, is essential for normal platelet function and hemostasis.