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Decreased in vitro fertilization and cleavage rates after an equipment error during CO(2) calibration
1Clinical Research Unit for Reproductive Medicine, Shanxi Provincial Hospital, Taiyuan, People's Republic of China. hxwang@public.ty.sx.cn
Objective:
To report the decreased IVF rate of oocytes and the reduced cleavage rate of pronuclear stage embryos after gas has backflowed from a gas analyzer into an incubator during the calibration of the CO(2) concentration.
Design:
Case report.
Setting:
Clinical research unit for reproductive medicine in a hospital.
Intervention(S):
Backflow of gas from a gas analyzer into an incubator during calibration of the CO(2) concentration.
Main Outcome Measure:
The IVF rate of oocytes and the cleavage rate of pronuclear stage embryos.
Result(S):
Thirty-two oocytes were retrieved from four infertile female patients. Nine oocytes were fertilized and four fertilized oocytes were cleaved. In a related animal experiment, the cleavage rate was 57.1% (36/63) in the control group and 25.4% (16/63) in the study group (P =.00026).
Conclusion(S):
The backflow of gas from the gas analyzer adversely affected the fertilization of oocytes and the cleavage of pronuclear stage embryos.