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Isolation and identification of bluetongue virus
A Clavijo1, R A Heckert, G C Dulac
1National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada. aclavijo@em.agr.ca
Journal of Virological Methods
|June 17, 2000
Summary
Detecting bluetongue virus (BTV) involves isolating the virus, amplifying it in embryonating chicken eggs and cell cultures, and then identifying it using specific assays. This multistep process ensures accurate identification and typing of BTV in infected animals.
Area of Science:
- Veterinary Virology
- Arthropod-borne Viruses
- Orbivirus Research
Background:
- Bluetongue virus (BTV) is a significant arthropod-borne orbivirus affecting livestock like sheep and cattle.
- Accurate detection and specific identification of BTV are crucial for disease control and management.
- Current diagnostic methods require a complex, multistep approach for virus isolation and characterization.
Purpose of the Study:
- To outline the established multistep process for the detection and specific identification of bluetongue virus (BTV).
- To detail the sequential stages involved in isolating, amplifying, and typing BTV from infected animal samples.
Main Methods:
- Virus isolation from animal blood or tissues.
- Amplification in embryonating chicken eggs (ECE) and subsequent passage into BHK-21 cell culture.
- Identification using immunoperoxidase assay with group-specific monoclonal antibodies and virus typing via neutralization tests.
Main Results:
- The described methodology allows for the isolation and amplification of BTV.
- The immunoperoxidase assay enables group-specific identification of the viral isolate.
- Virus neutralization tests provide serotype-specific typing of the bluetongue virus.
Conclusions:
- The multistep diagnostic process, from initial isolation to final typing, is effective for identifying and characterizing bluetongue virus.
- This comprehensive approach is essential for accurate bluetongue diagnosis in veterinary settings.
- Further refinement of these techniques can aid in understanding BTV epidemiology and developing targeted control strategies.