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Early life humoral response of ducks to DNA immunization against hepadnavirus large envelope protein
C Rollier1, C Charollois, C Jamard
1Unité de Recherche sur les Virus des Hépatites et les Pathologies Associées, Institut National de la Santé et de la Recherche Médicale unité 271, Lyon, France.
Abstract:
DNA vaccination may represent an interesting strategy for early life immunization. However, in some cases, this approach has been shown to induce a tolerance rather than immunity. We have compared the efficiency of neonatal DNA or protein immunization against hepadnavirus envelope protein using the duck hepatitis B virus (DHBV) model. Three-day-old ducklings were immunized with either a plasmid encoding the DHBV pre-S/S large envelope protein (L), or a recombinant preS protein, followed by sequential DNA or protein boosts at weeks 4 and 15. Our results showed that genetic immunization of duck neonates induced specific humoral response to DHBV L protein. Interestingly, an enhanced antibody response was elicited when animals received DNA priming-DNA boosting as compared to DNA priming-protein boosting.