Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Poly(ADP-ribose) polymerase-1: what have we learned from the deficient mouse model?

S Shall1, G de Murcia

  • 1UPR 9003 du Centre National de la Recherche Scientifique, Laboratoire conventionné avec le Commissariat à l'Energie Atomique, Ecole Supérieure de Biotechnologie de Strasbourg, France. sydney.shall@kcl.ac.uk

Mutation Research
|June 17, 2000
PubMed
Summary

Poly (ADP-ribose) polymerase-1 (PARP-1) is crucial for DNA repair and cellular response to genotoxic stress. PARP-1 deficiency impacts cell death and inflammation, with PARP-2 also playing a genome protection role.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel form of ataxia oculomotor apraxia characterized by oxidative stress and apoptosis resistance.

Cell death and differentiation·2007
Same author

Ionising radiation induces the expression of PARP-1 and PARP-2 genes in Arabidopsis.

Molecular genetics and genomics : MGG·2001
Same author

Normal telomere length and chromosomal end capping in poly(ADP-ribose) polymerase-deficient mice and primary cells despite increased chromosomal instability.

The Journal of cell biology·2001
Same author

Loss of poly(ADP-ribose) polymerase-1 causes increased tumour latency in p53-deficient mice.

The EMBO journal·2001
Same author

Early embryonic lethality in PARP-1 Atm double-mutant mice suggests a functional synergy in cell proliferation during development.

Molecular and cellular biology·2001
Same author

Werner's syndrome T lymphocytes display a normal in vitro life-span.

Mechanisms of ageing and development·2001

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Poly (ADP-ribose) polymerase-1 (PARP-1) is a key enzyme in the DNA damage surveillance network.
  • It recognizes and is activated by DNA strand breaks, playing a role in base excision repair (BER).

Purpose of the Study:

  • To review recent data on PARP knockout mice and chemical inhibitors.
  • To explore the caretaker function of PARP-1 and the role of PARP-2.

Main Methods:

  • Generation and analysis of three independent PARP-1 deficient mouse models.
  • Comparison with chemical inhibitor approaches.

Main Results:

  • PARP-1 knockout mice confirmed its caretaker function under genotoxic stress.

Related Experiment Videos

  • PARP-1 deficiency revealed roles in ischemia-reperfusion injury and inflammation.
  • PARP-2 was identified as a novel DNA damage-dependent poly ADP-ribose polymerase.

    • Conclusions:

    • PARP-1 is essential for mammalian cell protection against genotoxic stress.
    • PARP-1 influences cell death and inflammatory processes.
    • The PARP family, including PARP-2, is involved in genome protection.