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Related Experiment Videos

Human umbilical vein endothelial cells express multiple prolactin isoforms.

A M Corbacho1, Y Macotela, G Nava

  • 1Centro de Neurobiología, Universidad Nacional Autónoma de México, 76001, Querétaro, Qro, México.

The Journal of Endocrinology
|June 17, 2000
PubMed
Summary

Prolactin (PRL) gene is expressed in human umbilical vein endothelial cells (HUVEC), producing various PRL isoforms. Unlike in bovine cells, these HUVEC-derived PRLs show minimal bioactivity and can stimulate proliferation.

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Area of Science:

  • Endocrinology
  • Cell Biology
  • Vascular Biology

Background:

  • Prolactin (PRL) hormonal family members influence endothelial cell functions.
  • PRL isoforms may act as autocrine regulators in endothelial cells.
  • Bovine brain capillary endothelial cells (BBCEC) express the PRL gene, and anti-PRL antibodies inhibit their proliferation.

Purpose of the Study:

  • To investigate the expression of PRL gene and its isoforms in human umbilical vein endothelial cells (HUVEC).
  • To compare the bioactivity and functional effects of PRL in HUVEC with those in BBCEC.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) to detect PRL mRNA.
  • Immunocytochemistry and metabolic labeling immunoprecipitation to identify PRL proteins.

Related Experiment Videos

  • Nb2 cell proliferation bioassay to determine PRL bioactivity.

    • Main Results:

    • HUVEC express full-length PRL mRNA and a smaller PRL transcript.
    • PRL immunoreactive proteins of various molecular masses (60, 23, 21, 16, and 14 kDa) were detected in HUVEC.
    • HUVEC-conditioned media exhibited low PRL bioactivity, and anti-PRL antibodies stimulated HUVEC proliferation.

    Conclusions:

    • The PRL gene is expressed in human umbilical vein endothelial cells, similar to bovine brain capillary endothelial cells.
    • Endothelium from different species and vessel types share PRL gene expression.
    • The autocrine role of expressed PRL isoforms may differ between endothelial cell types.