Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet aggregation by hepatitis B surface antigen-antibody complexes.

H Daugharty, R Gogel

    Infection and Immunity
    |September 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Chlamydia DNA extraction for use in PCR: stability and sensitivity in detection.

    Journal of clinical laboratory analysis·1998
    Same author

    Application of a nested, multiplex PCR to psittacosis outbreaks.

    Journal of clinical microbiology·1997
    Same author

    ELISPOT assay for Chlamydia-specific, antibody-producing cells correlated with conventional complement fixation and microimmunofluorescence.

    Journal of clinical laboratory analysis·1997
    Same author

    Utility of complement fixation and microimmunofluorescence assays for detecting serologic responses in patients with clinically diagnosed psittacosis.

    Journal of clinical microbiology·1994
    Same author

    Morphologic and staining characteristics of a cyanobacterium-like organism associated with diarrhea.

    The Journal of infectious diseases·1991
    Same author

    Comparative study with in situ hybridization and immunocytochemistry in detection of HIV-1 in formalin-fixed paraffin-embedded cell cultures.

    Journal of clinical laboratory analysis·1990

    Hepatitis B antigen-antibody complexes were detected using platelet aggregation, showing higher incidence in antigen-positive sera. This method offers insights into hepatitis B virus (HBV) immune complex formation and anti-complementary activity.

    Area of Science:

    • Immunology
    • Virology
    • Hematology

    Background:

    • Hepatitis B virus (HBV) infection is a significant global health concern.
    • Detecting hepatitis B surface antigen (HBsAg) and antibody (HBsAb) is crucial for diagnosis and management.
    • Antigen-antibody complex formation plays a role in immune responses.

    Purpose of the Study:

    • To investigate the utility of platelet aggregation as an indicator of hepatitis B antigen-antibody complex formation.
    • To compare the incidence of HBsAg-HBsAb complexes in HBsAg-positive versus HBsAb-positive sera.
    • To assess the correlation between platelet aggregation, radioimmunoassay (RIA) values, and anti-complementary activity.

    Main Methods:

    • Serum specimens positive for HBsAg or HBsAb by RIA were tested for platelet aggregation.

    Related Experiment Videos

  • Platelet aggregation titers were determined.
  • Anti-complementary activity was assessed in platelet aggregation-positive and -negative sera.
  • Inhibition studies involved preincubation with additional HBsAg, HBsAb, or anti-IgG serum.

    • Main Results:

    • Platelet aggregation was observed upon mixing HBsAg and HBsAb, indicating complex formation.
    • HBsAg-positive sera showed a higher incidence of platelet aggregation compared to HBsAb-positive sera.
    • An inverse correlation between platelet aggregation titers and RIA values was significant for HBsAg sera.
    • Platelet aggregation-positive sera exhibited higher anti-complementary activity, particularly HBsAg sera.
    • Preincubation with additional HBsAg inhibited platelet aggregation, while anti-IgG enhanced it in HBsAg sera.

    Conclusions:

    • Platelet aggregation serves as a sensitive indicator for detecting HBsAg-antibody complexes.
    • The findings suggest distinct characteristics of immune complexes in HBsAg-positive versus HBsAb-positive sera.
    • Platelet aggregation assays may provide complementary information to RIA in hepatitis B diagnostics.