Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell adhesive sequences in mouse laminin beta1 chain.

M Nomizu1, Y Kuratomi, M L Ponce

  • 1Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, Bethesda, Maryland 20892, USA. nomizu@ees.hokudai.ac.j

Archives of Biochemistry and Biophysics
|June 22, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thymosin β4 Promotes Dermal Healing.

Vitamins and hormones·2016
Same author

Thymosin Beta 4: A Potential Novel Therapy for Neurotrophic Keratopathy, Dry Eye, and Ocular Surface Diseases.

Vitamins and hormones·2016
Same author

Effects of amelogenins on angiogenesis-associated processes of endothelial cells.

Journal of wound care·2011
Same author

Advancing science and technology via 3D culture on basement membrane matrix.

Journal of cellular physiology·2009
Same author

Preparation of basement membrane components from EHS tumors.

Current protocols in cell biology·2008
Same author

Preparation of gelled substrates.

Current protocols in cell biology·2008

Researchers identified multiple cell adhesion sites on the laminin beta1 chain. Some sites show cell-type specificity, with key sequences clustered in the amino-terminal globular domain, crucial for cell binding.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Extracellular Matrix Research

Background:

  • Laminin-1 is a key basement membrane protein composed of alpha1, beta1, and gamma1 chains.
  • Understanding laminin chain functions is crucial for cell adhesion and tissue development research.

Purpose of the Study:

  • To identify specific cell adhesive sequences within the mouse laminin beta1 chain.
  • To investigate the cell-type specificity of these identified adhesive sequences.

Main Methods:

  • Synthesized 187 overlapping peptides covering the entire mouse laminin beta1 chain.
  • Tested binding of HT-1080 fibrosarcoma and B16-F10 melanoma cells to peptides.
  • Utilized peptide-conjugated Sepharose beads and peptide-coated plates for adhesion assays.
  • Investigated inhibition by EDTA and heparin to characterize binding mechanisms.

Related Experiment Videos

Main Results:

  • Fourteen peptides demonstrated significant cell adhesive activity.
  • Potent cell attachment was observed for peptides B-7, B-133, and B-160.
  • Cell binding to specific peptides was inhibited by EDTA and heparin, indicating distinct mechanisms.
  • Eight active peptides are clustered on the amino-terminal globular domain of the beta1 chain.

Conclusions:

  • The laminin beta1 chain contains multiple functional sites for cell adhesion.
  • These sites exhibit cell-type specificity, influencing interactions with various cell types.
  • The amino-terminal globular domain of laminin beta1 plays a significant role in mediating cell binding.