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[Influence of HBV/C mutation on HLA expression of host cells]
Objective:
To study the influence of HBV/C mutation on host cellular HLA expression.
Methods:
HBV expression vectors carrying wild or variant HBV/C gene were constructed and transferred into HepG(2) cells. HBV/C gene expression on the host cells was identified. The expression of HLA-I and HLA-DR on the host cell membrane was detected.
Results:
DNA segments similar with HBV/C gene size and HBcAg were detected by PCR and Western blot analysis, respectively. Almost all HepG(2) cells expressed HLA-I but not HLA-DR. The fluorescence intensity of HLA-I expression on host cells was different: HepG(2) was 57.8 and wild vector 54.3. The wild HBV/C gene declined significantly to 31.2. While V60, G87 and L97 increased to 43.0, 54.0 and 69.4, respectively. L97 was greatly increased with 11.6 higher than HepG(2). HLA-DR had no significant expression.
Conclusion:
Replication and expression of HBV/C gene can influence HLA-I expression on the host cells directly. Influence of variant gene on HLA-I expression is different from the wild gene. This might relate with the exacerbation of diseases resulting from HBV mutation.