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Determination of Ibuprofen in human plasma by high-performance liquid chromatography: validation and application in
R Canaparo1, E Muntoni, G P Zara
1Department of Anatomy, Pharmacology and Forensic Medicine, University of Torino, Torino, Italy. canaparo@medfarm.unito.it
Abstract:
A specific method for the simultaneous determination of S-(+)Ibuprofen and R-(-)Ibuprofen enantiomers in human plasma is described. Adopting a high-performance liquid chromatographic (HPLC) system with spectrofluorometer detector, the compounds were extracted from plasma in alcohol medium and were separated on C18 column, using a solution of acetonitrile-water-acetic acid-triethylamine as mobile phase. The limit of quantitation was 0.1 microg/mL for both compounds. The method was validated by intra-day assays at three concentration levels and was used in a kinetic study in healthy volunteers. During the study we carried out inter-day assays to confirm the feasibility of the method.