Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD44 expression and regulation during mammary gland development and function.

L Hebbard1, A Steffen, V Zawadzki

  • 1Forschungszentrum Karlsruhe, Institute für Toxikologie und Genetik, Postfach 3640, D-76021 Karlsruhe, Germany.

Journal of Cell Science
|June 23, 2000
PubMed
Summary

CD44v6 epitope expression in mammary glands is linked to stem cells and follows hormonal cycles. Its presence in breast tumors may reflect stem cell origin and differentiation status.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy and safety of mepolizumab in hypereosinophilic syndrome.

European annals of allergy and clinical immunology·2026
Same author

Experiences from patients in mental healthcare accessing their electronic health records: results from a cross-national survey in Estonia, Finland, Norway, and Sweden.

BMC psychiatry·2024
Same author

Refined high-content imaging-based phenotypic drug screening in zebrafish xenografts.

NPJ precision oncology·2023
Same author

Use of drug-induced sleep endoscopy in Germany-an analysis based on claims data.

Somnologie : Schlafforschung und Schlafmedizin = Somnology : sleep research and sleep medicine·2023
Same author

Temperature dependence studies of tissue-mimicking phantoms for ultra-wideband microwave breast tumor detection.

Biomedical physics & engineering express·2022
Same author

The descending pain modulation system predicts short term efficacy of multimodal pain therapy: an observational prospective cohort study.

Postgraduate medicine·2021

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Cancer Biology

Background:

  • The CD44v6 epitope is found in human mammary carcinomas, but its prognostic value and function remain unclear.
  • Understanding CD44v6 expression in normal mammary glands is crucial for interpreting its role in tumors.

Purpose of the Study:

  • To investigate the normal postnatal expression patterns and regulation of the CD44v6 epitope in murine mammary glands.
  • To determine the relationship between CD44v6 expression, mammary stem cells, and hormonal influences.

Main Methods:

  • Analysis of CD44v6 epitope expression in murine mammary glands across different physiological states (puberty, estrous cycle, lactation, involution).
  • Utilized HC11 mammary epithelial cell line and cloned murine CD44 promoter to study transcriptional regulation.

Related Experiment Videos

  • Investigated the role of growth factors (IGF-1, EGF) and hormones in modulating CD44 transcription.

    • Main Results:

    • Significant CD44v6 expression begins at puberty and fluctuates with the estrous cycle, observed in myoepithelial and luminal cells.
    • Expression is downregulated during lactation and re-emerges during involution.
    • Growth factors and hormones were shown to modulate CD44 transcription in mammary epithelial stem cells.

    Conclusions:

    • CD44v6 epitope is expressed in mammary epithelial stem cells and their derivatives, with expression regulated by hormones and growth factors.
    • Aberrant CD44v6 expression in mammary tumors may indicate perturbed growth factor/hormone signaling.
    • CD44v6 expression in breast tumors correlates with the stem cell origin and differentiation state of the tumor cells.