Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vivo DNA damage in gastric epithelial cells.

S M Everett1, K L White, C J Schorah

  • 1The Centre for Digestive Diseases, The General Infirmary at Leeds, UK. medsmev@stjames.leeds.ac.uk

Mutation Research
|June 23, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disruption of gut barrier integrity and host-microbiome interactions underlie MASLD severity in patients with type-2 diabetes mellitus.

Gut microbes·2024
Same author

A multi-national survey of experience and attitudes towards commencing home parenteral nutrition for patients with advanced cancer.

Clinical nutrition ESPEN·2022
Same author

Offshore pelagic subsidies dominate carbon inputs to coral reef predators.

Science advances·2021
Same author

Organization of Chemically Activated Food Search Behavior in Procambarus clarkii Girard and Orconectes rusticus Girard Crayfishes.

The Biological bulletin·2017
Same author

Management of Helicobacter pylori infection-the Maastricht V/Florence Consensus Report.

Gut·2016
Same author

Alpha-thalassemia intellectual disability: variable phenotypic expression among males with a recurrent nonsense mutation - c.109C>T (p.R37X).

Clinical genetics·2014
Same journal

Monoallelic germline RAD51C, RAD51D, and BRIP1 variants in hereditary cancer testing: Variant spectrum and clinical counselling implications.

Mutation research·2026
Same journal

Prediction of hepatocellular carcinoma associated biomarkers in TP53 gene; A comprehensive in silico analysis.

Mutation research·2026
Same journal

IDH1 mutation promotes angiogenesis via upregulation of hypoxia inducible factor 1 alpha in glial tumors.

Mutation research·2026
Same journal

Targeting overexpressed oncogenes in esophageal cancer through miRNA-mediated gene silencing: Insights from binding affinity and thermodynamic profiling.

Mutation research·2026
Same journal

The active compound quercetin from Polygonum cuspidatum targets COL3A1 to enhance CD8⁺ T cell cytotoxicity in gastric cancer.

Mutation research·2026
Same journal

E2F1 promotes LIHC malignant phenotype via NEK2-mediated Wnt/β-catenin and Notch activation and EMT.

Mutation research·2026
See all related articles

The comet assay effectively measures DNA damage in human gastric epithelial cells, revealing that damage increases in older cells and can be induced by oxidative stress.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer risk factors are known, but DNA damage in gastric epithelial cells is understudied.
  • The comet assay is a valuable tool for assessing DNA damage in individual cells.

Purpose of the Study:

  • Validate the comet assay for human gastric biopsies.
  • Optimize digestion conditions for epithelial cell release.
  • Investigate DNA damage influenced by oxidative stress and cell depth.

Main Methods:

  • Human gastric biopsies were digested using pronase, collagenase, and EDTA.
  • Cell suspensions were analyzed for cell concentration and purity.
  • The comet assay quantified DNA damage (comet %) under various conditions, including hydrogen peroxide exposure.

Related Experiment Videos

Main Results:

  • Pronase and collagenase yielded optimal digestion, releasing predominantly epithelial cells.
  • The comet assay demonstrated high reproducibility (inter-observer 6.1%, inter-assay 4.5%).
  • DNA damage significantly increased with oxidative stress (H(2)O(2)) and was lower in cells from deeper gastric crypts.

Conclusions:

  • The comet assay is a reliable method for quantifying DNA damage in gastric epithelial cells.
  • DNA damage accumulates in older, superficial gastric cells.
  • Oxidative stress is a significant factor in inducing DNA damage in these cells.