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Updated: Jul 16, 2026

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
Published on: January 19, 2011
[The quantitative patterns of the modified one-center bioreceptor model]
1N. N. Semenov Institute of Chemical Physics, Russian Academy of Science, Moscow, Russia. poltorakov@mtu-net.ru
Abstract:
A modified one site model of the bioreceptor have been used to estimate quantitatively the phenomenon of the full agonism. Threshold phenomenon, spare receptors and linear dependence of the biological effect on concentration of complex agonist-receptor has been determinate by the general correlation equations. The equations of one site model provides a good fit to the experimental curves "dose-response" for the full agonists and allows to calculate the value of space receptors. The model includes occupancy Clark's theory and law "all or nothing". The interaction of acetylcholine and aklyltrimethylammonium salts with muscarinic acetylholine receptors is analysed as an example of use of this equation.
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