Related Experiment Video

Updated: Oct 13, 2026

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
08:31

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus

J D Gangemi, D G Sharp

    Journal of Virology
    |October 1, 1976
    PubMed

    Insights

    Restriction endonuclease Hind III fragmented vaccinia DNA into 14 pieces. Analysis revealed minor molecular differences between vaccinia strains, with the genome averaging 125 x 10^6 molecular weight.

    Area of Science:

    • Molecular Biology
    • Virology
    • Biochemistry

    Background:

    • Restriction endonucleases are crucial tools for DNA analysis.
    • Vaccinia virus DNA has been extensively studied but requires precise mapping.

    Purpose of the Study:

    • To analyze vaccinia virus DNA structure using Hind III.
    • To determine the molecular weight of DNA fragments generated by Hind III digestion.
    • To compare DNA from different vaccinia virus strains.

    Main Methods:

    • Digestion of vaccinia DNA with Haemophilus influenzae restriction endonuclease (Hind III).
    • Separation and analysis of DNA fragments using gel electrophoresis.
    • Calculation of fragment molecular weights and genome size.

    Main Results:

    • Hind III cleaved vaccinia DNA into 14 distinct fragments.
    • Fragment molecular weights ranged from 0.5 x 10^6 to 30 x 10^6.
    • A small molecular difference was observed in one fragment between WR and CV-1 vaccinia strains.
    • The average molecular weight of the vaccinia genome was calculated to be 125 x 10^6.

    Conclusions:

    • Hind III provides a reliable method for fragmenting vaccinia DNA.
    • The vaccinia genome size is approximately 125 x 10^6.
    • Minor genomic variations exist between vaccinia virus strains.

    More Related Videos

    Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins09:27

    Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

    Published on: January 17, 2014

    Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection09:25

    Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

    Published on: May 24, 2020

    Related Concept Videos

    Viral Recombination00:57

    Viral Recombination

    Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
    Restriction Enzymes01:11

    Restriction Enzymes

    Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
    The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...