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Adenovirus late gene expression does not require a Rev-like nuclear RNA export pathway

C Rabino1, A Aspegren, K Corbin-Lickfett

  • 1Rudbeck Laboratory, Department of Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.

Journal of Virology
|June 23, 2000
PubMed

Insights

Adenovirus late mRNA export relies on viral proteins. However, a proposed pathway involving a specific protein

Area of Science:

  • Molecular biology
  • Virology
  • Cellular biology

Background:

  • Adenovirus late mRNA export is crucial for viral replication.
  • Viral early proteins, including a 34-kDa protein, are known to facilitate this process.
  • The 34-kDa protein possesses a leucine-rich nuclear export signal (NES), analogous to the HIV Rev protein.

Purpose of the Study:

  • To investigate the role of NES-mediated RNA export in adenovirus infection.
  • To determine if the proposed Rev-like NES-mediated pathway is essential for adenovirus late gene expression.

Main Methods:

  • Experimental analysis of adenovirus-infected cells.
  • Focus on the function of the 34-kDa viral protein and its NES.

Main Results:

  • The study found that NES-mediated RNA export is not essential for adenovirus late gene expression.
  • This challenges the proposed model of a Rev-like NES-mediated export pathway being critical.

Conclusions:

  • The Rev-like NES-mediated export pathway is not a critical mechanism for adenovirus late mRNA export.
  • Adenovirus likely utilizes alternative pathways for efficient late gene expression.

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