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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

Fluorescence in situ hybridization: a rapid analysis to verify chromosome aberrations

C Tocharoentanaphol1, R Parinayok, N Punthuwattana

  • 1Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.

Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
|June 24, 2000
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...