Related Experiment Video
Updated: Aug 14, 2026
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
Published on: December 28, 2015
Synthesis of glycosylated polyethylenimine with reduced toxicity and high transfecting efficiency
F Leclercq1, C Dubertret, B Pitard
1Laboratoire de Chimie Bioorganique et Biotechnologie Moleculaire et cellulaire, Unité Mixte ENSCP/CNRS/AVENTIS (UMR 7001), ENSCP, Paris, France. fralecle@ext.jussieu.fr
Abstract:
A safe and efficient synthesis of glycosylated polyethylenimine using titanium (IV) isopropoxide and sodium borohydride has been carried out as a substitute for the highly toxic sodium cyanoborohydride method currently used. Poryplexes formed between DNA and the various glycosylated polyethylenimines appeared to be much less cytotoxic than polyethylenimine (PEI)/DNA polyplexes.
