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Determination of histamine in tomatoes by liquid chromatography/mass spectrometry
E Bolygo1, P A Cooper, K M Jessop
1Zeneca Agrochemicals, Jealotts Hill Research Station, Bracknell, Berkshire, UK.
Journal of AOAC International
|June 27, 2000
Abstract:
The histamine level in tomato fruits and pastes was determined by 2 orthogonal techniques as a means of comparing accuracy. Close statistical agreement was found between assays for free histamine by capillary electrophoresis (with UV absorbance detection), and for the dansyl derivative by reversed-phase liquid chromatography (LC). Both techniques have adequate sensitivity for the analysis of endogenous histamine in tomatoes, but LC/electrospray tandem mass spectrometry was more sensitive by at least an order of magnitude, down to a level of 0.05 mg/kg.