Mouse B and T lymphocyte responses to purified timothy pollen antigens in vitro

Purified populations of splenic B and T lymphocytes from LAF mice immunized with a crude extract of timothy pollen (WST) responded specifically to pollen antigens in an in vitro lymphocyte transformation system. The peak lymphocyte transformation response occurred 5 days after a secondary immunization and was the result of T-B cell cooperation in vitro. Wtih two purified pollen antigens as in vitro stimulants we were able to define at least two antigen-specific population of B cells and one population of T cells. These results were confirmed by inhibition studies with a monovalent hapten from WST, Antigen D.