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Related Experiment Videos

Progress in packed column supercritical fluid chromatography: materials and methods.

C F Poole1

  • 1Wayne State University, Department of Chemistry, Rm. 171 Chemistry, Detroit, MI 48202, USA. cfp@chem.wayne.edu

Journal of Biochemical and Biophysical Methods
|June 28, 2000
PubMed
Summary
This summary is machine-generated.

Packed column supercritical fluid chromatography (SFC) offers enhanced separation efficiency and speed compared to normal-phase HPLC. It is increasingly used for analytical and preparative separations, especially for racemic mixtures.

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Silica-based chemically bonded sorbents are common in packed column supercritical fluid chromatography (SFC).
  • Additives and solvent-modified fluids are used to improve peak shape by suppressing solute-sorbent interactions.

Purpose of the Study:

  • To summarize recent advancements in packed column supercritical fluid chromatography.
  • To highlight the advantages of packed column SFC over normal-phase HPLC.

Main Methods:

  • Utilizing silica-based chemically bonded sorbents.
  • Employing solvent-modified fluids with additives.
  • Implementing composition programming for gradient elution.

Main Results:

  • Packed column SFC provides faster separations and higher efficiencies than normal-phase HPLC.
  • It allows for faster column re-equilibration and a broader range of optimization variables.
  • Composition programming is effective for gradient elution in SFC due to fluid compressibility.

Conclusions:

  • Packed column SFC is a powerful technique for separating mixtures typically analyzed by normal-phase HPLC.
  • Its benefits include speed, efficiency, and versatility.
  • It is increasingly favored for analytical and preparative separation of racemic compounds using enantioselective sorbents.