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Cyclin D and cyclin E expression in normal and adenomatous pituitary
S Jordan1, K Lidhar, M Korbonits
1Department of Histopathology, St Bartholomew's Hospital, London EC1A 7BE, UK.
European Journal of Endocrinology
|June 28, 2000
Summary
Cyclin D1 is over-expressed in aggressive pituitary tumors, while cyclin E is increased in Cushing's disease. This study investigated cyclin D1 and E expression in 95 pituitary tumors.
Area of Science:
- Endocrinology
- Oncology
- Cell Biology
Background:
- Cyclins regulate cell cycle progression.
- Cyclin overexpression is common in various tumors.
- Pituitary adenomas, a frequent endocrine neoplasia, lack extensive study regarding cyclin D1 and E expression.
Purpose of the Study:
- To investigate the expression of cyclin D1 and cyclin E in pituitary tumors.
- To correlate cyclin expression with different pituitary tumor subtypes and normal pituitary tissue.
Main Methods:
- Immunohistochemical staining for cyclin D1 and cyclin E on 95 pituitary tissues (normal and tumors).
- Quantification of staining by counting 500 cells per tissue.
- Blinded analysis and statistical comparison between tissue groups.
Main Results:
- Cyclin D1 nuclear staining was more frequent in non-functioning and aggressive pituitary tumors compared to other types and normal pituitary.
- Cyclin E expression was specifically increased in corticotroph tumors from patients with Cushing's disease.
- No cytoplasmic staining for cyclin D1 was observed.
Conclusions:
- Cyclin D1 overexpression is associated with aggressive and non-functioning pituitary tumors.
- Increased cyclin E expression is linked to Cushing's disease.
- Elevated cyclin E in Cushing's disease may correlate with previously reported low p27 protein expression.