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Related Experiment Videos

X inactivation-specific transcript expression in mouse oocytes and zygotes.

R Avner1, J Wahrman, C Richler

  • 1Unit for Development of Molecular Biology and Genetic Engineering, The Hebrew University, Hadassah Medical School, Jerusalem.

Molecular Human Reproduction
|June 29, 2000
PubMed
Summary

XIST gene expression occurs in unfertilized mouse oocytes and zygotes, preceding earlier known expression in 4-cell embryos. This suggests XIST transcripts are present from the earliest stages of development.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Epigenetics

Background:

  • The X inactivation-specific transcript (XIST) gene is crucial for X chromosome inactivation in female mammals.
  • Previous studies detected XIST expression in 4-cell stage mouse embryos, with maternal imprinting followed by random expression in later embryonic lineages.
  • The precise timing of XIST expression during early oogenesis and fertilization remained unclear.

Purpose of the Study:

  • To investigate the expression of the XIST gene in unfertilized mouse oocytes and pronuclear stage zygotes.
  • To determine if XIST expression precedes the 4-cell stage in mouse embryonic development.
  • To explore potential differences in XIST expression patterns in early zygotes.

Main Methods:

  • In-situ reverse transcription-polymerase chain reaction (RT-PCR) was employed to detect XIST gene expression.

Related Experiment Videos

  • Analysis was performed on unfertilized mouse oocytes and pronuclear stage zygotes.
  • Expression patterns were visualized and characterized.

    • Main Results:

    • XIST gene expression was detected in unfertilized mouse oocytes.
    • XIST transcripts were also found in pronuclear stage zygotes, including both XX and XY embryos.
    • Distinct expression patterns (rod-like vs. rounded punctate signals) were observed in pronuclear stage embryos.

    Conclusions:

    • XIST gene expression initiates earlier than previously thought, present in oocytes and zygotes.
    • XIST transcripts may be present in both male and female zygotes, challenging initial assumptions about its role.
    • These findings align with early XIST expression observed in human embryos, suggesting conserved mechanisms.