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Simultaneous detection of hepatitis B, C, and G viral genomes by multiplex PCR method
N Konomi1, M Yamaguchi, H Naito
1Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Japanese Journal of Infectious Diseases
|June 29, 2000
Abstract:
We established a multiplex polymerase chain reaction (PCR) method for simultaneous detection of hepatitis B, C, and G viral genomes. The levels of concordance with the data obtained by conventional single PCR method were 100% for single infection, 98 to 100% for double infections, and 92% for triple infections. This method is not only suited to rapid, large-scale epidemiological screening and clinical diagnosis of those virus infections occurring alone or in combination, but is also time- and cost-effective.