Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear volume estimation using different sampling, measurement and calculation methods.

C Xia1, Z Xia

  • 1Department of Histology and Embryology, Medical College, Jinan University, Guangzhou, People's Republic of China. lyxzp@jnu.edu.cn

Analytical and Quantitative Cytology and Histology
|June 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physiological responses of Robinia pseudoacacia L. of different origins to soil nitrogen supply.

Plant biology (Stuttgart, Germany)·2026
Same author

Prediction and parameter inversion of wavelength-tunable fiber laser based on machine learning.

Applied optics·2026
Same author

Enhancing Precision in Ductal Carcinoma In Situ Radiotherapy: Integrating Biomarkers, Monitoring, and Stratified Care.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

[Spiradenoma with adenoid cystic carcinoma-like changes: a clinicopathological and genetic molecular analysis of three cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2025
Same author

[Risk of and response to cross-border importation and secondary transmission of malaria].

Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control·2025
Same author

Investigation on body condition score, milk yield, reproductive performance, and health of dairy cows in four intensive dairy farms during lactation cycle.

Polish journal of veterinary sciences·2025
Same journal

Micronuclei and other nuclear anomalies in buccal mucosa following exposure to X-ray radiation.

Analytical and quantitative cytology and histology·2012
Same journal

Color deconvolution. Optimizing handling of 3D unitary optical density vectors with polar coordinates.

Analytical and quantitative cytology and histology·2012
Same journal

Nuclear morphometry in the differential diagnosis of eosinophilic renal epithelial tumors.

Analytical and quantitative cytology and histology·2012
Same journal

Spectrum of prostatic nonepithelial tumors and tumorlike conditions with spindle cell features.

Analytical and quantitative cytology and histology·2012
Same journal

A framework for quantitative assessment of Ki67 distribution in preneoplastic bronchial epithelial lesions.

Analytical and quantitative cytology and histology·2012
Same journal

Classification and grading of noninvasive and invasive neoplasms of the urothelium.

Analytical and quantitative cytology and histology·2012
See all related articles

Accurate nuclear volume measurement requires careful selection of sampling, preparation, and calculation methods. Different techniques yield varying results, emphasizing the need for standardized protocols in stereology and image analysis.

Area of Science:

  • Stereology
  • Cell Biology
  • Quantitative Histology

Background:

  • Accurate measurement of nuclear volume is crucial for understanding cell function and pathology.
  • Existing methods for volume estimation from tissue sections vary in their reliability.

Purpose of the Study:

  • To evaluate the reliability of nuclear volume parameters derived from different sampling, measurement, and calculation techniques.
  • To identify optimal methods for reproducible nuclear volume quantification.

Main Methods:

  • Captured images of primary spermatocyte and spermatoblast nuclei from seminiferous tubule sections using high (100x) and low (20x) magnification objectives.
  • Measured and calculated nuclear volumes using five distinct methods within an image analysis system.

Related Experiment Videos

Main Results:

  • Nucleator and area equivalent diameter methods yielded similar nuclear volume estimates on largest profiles.
  • Random Feret diameter and equivalent diameter methods resulted in significantly larger volumes.
  • Volumes derived from tissue images were lower than those from largest nuclear profiles due to slice variation.
  • Shape factors were consistent across different magnifications.

Conclusions:

  • Reproducible nuclear volume parameters necessitate appropriate sample preparation, sampling strategies, and calculation formulas tailored to nuclear morphology.
  • Standardization of stereological methods is essential for reliable nuclear volume quantification.