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Updated: Oct 15, 2026

Isolation of Leukocytes from the Human Maternal-fetal Interface
Published on: May 21, 2015
The role of amniotic fluid interleukin-6, and cell adhesion molecules, intercellular adhesion molecule-1 and
C D Hsu1, K Aversa, E Meaddough
1Department of OB/GYN, Yale University School of Medicine, New Haven, CT 06525, USA. chaur-dong.hsu@yale.edu
Problem:
To determine amniotic fluid concentrations and correlations of interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1), and leukocyte adhesion molecule-1 (LAM-1) in patients with and without intra-amniotic infection.
Method Of Study:
Fourteen specimens with intra-amniotic infection and 45 without intra-amniotic infection were studied. Intra-amniotic infection was defined as the presence of a positive amniotic fluid culture. Amniotic fluid IL-6, ICAM-1, and LAM-1 levels were determined by an enzyme-linked immunoassay, and normalized by amniotic fluid creatinine levels.
Results:
Amniotic fluid concentrations of IL-6 and LAM-1 were significantly higher in patients with than without intra-amniotic infection. However, amniotic fluid ICAM-1 concentrations were not significantly different between two groups. Amniotic fluid IL-6, LAM-1, and ICAM-1 were positively correlated.
Conclusions:
Our data indicate that amniotic fluid IL-6 is significantly associated with an increased adhesion molecule expression in intra-amniotic infection. However, LAM-1 plays a more important role than ICAM-1 in intra-amniotic infection.
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