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Regulated expression of endothelial cell-derived lipase
K Hirata1, T Ishida, H Matsushita
1First Department of Internal Medicine, Kobe University School of Medicine, Japan.
Biochemical and Biophysical Research Communications
|June 29, 2000
Summary
Endothelial-derived lipase (EDL) expression increases with inflammation and mechanical stress in blood vessel cells. This suggests EDL plays a key role in regulating vessel wall lipid metabolism and may impact vascular diseases like atherosclerosis.
Area of Science:
- Biochemistry
- Molecular Biology
- Vascular Biology
Background:
- A novel lipoprotein lipase-like gene, endothelial-derived lipase (EDL), was identified in endothelial cells.
- In vitro studies indicate EDL possesses phospholipase activity.
- Preliminary in vivo data suggest EDL's involvement in high-density lipoprotein metabolism regulation.
Purpose of the Study:
- To investigate the local regulation of EDL expression and activity within the blood vessel wall.
- To understand how EDL expression is controlled in human endothelial cells.
Main Methods:
- Examined EDL mRNA levels in cultured human umbilical vein and coronary artery endothelial cells.
- Assessed the impact of inflammatory cytokines (TNF-alpha, IL-1beta) on EDL mRNA.
- Investigated the effects of fluid shear stress and cyclic stretch on EDL mRNA expression.
Main Results:
- EDL mRNA levels were significantly upregulated by inflammatory cytokines TNF-alpha and IL-1beta in both endothelial cell types.
- Both fluid shear stress and cyclic stretch treatments led to increased EDL mRNA levels.
- These findings demonstrate a highly regulated expression pattern for EDL in vascular endothelial cells.
Conclusions:
- The expression of endothelial-derived lipase (EDL) is tightly controlled by inflammatory and mechanical stimuli in vascular endothelial cells.
- EDL is likely integral to modulating lipid metabolism within the vessel wall.
- EDL may contribute to the pathogenesis of vascular diseases, including atherosclerosis.