Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple copies of beta-lactoglobulin promoter do not function as LCR.

R M James1, C Neil, J Webster

  • 1Division of Molecular Biology, Roslin Institute (Edinburgh), Midlothian, United Kingdom.

Biochemical and Biophysical Research Communications
|June 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and MRI variables in decision support systems for prostate MRI: A systematic review of decision support tools, nomograms, and risk models.

Radiography (London, England : 1995)·2025
Same author

Survival outcomes in patients with sigmoid volvulus.

International journal of colorectal disease·2025
Same author

Seasonal variations regarding incidence of CTPA confirmed pulmonary embolism in Belfast, Northern Ireland, from 2014 to 2022.

The Ulster medical journal·2024
Same author

The Binghampton Homœopathic Medical Association.

The Homoeopathic physician·2023
Same author

Expanding the scope of Endocrine Connections.

Endocrine connections·2023
Same author

Arrhythmias and Their Electrophysiological Mechanisms in Takotsubo Syndrome: A Narrative Review.

Heart, lung & circulation·2022
Same journal

Necrotic tumor cells contribute to pro-tumoral responses and inflammatory mediator production via NF-κB activation in oral squamous cell carcinoma.

Biochemical and biophysical research communications·2026
Same journal

GPR4 promotes ferroptosis in nucleus pulposus cells via MAPK-dependent mitophagy suppression.

Biochemical and biophysical research communications·2026
Same journal

Residue interaction chains facilitating the structural change of ND6 subunit in respiratory complex I.

Biochemical and biophysical research communications·2026
Same journal

CD206 deficiency aggravates paraquat-induced acute lung injury in mice.

Biochemical and biophysical research communications·2026
Same journal

Synthesising lithium-alumina-borate glass-ceramic for potential applications in regenerative bone establishment.

Biochemical and biophysical research communications·2026
Same journal

A coelenterazine-dependent luciferase from the deep-sea coral Anthoptilum murrayi.

Biochemical and biophysical research communications·2026
See all related articles

Adding more transcription factor binding sites to DNA constructs did not enhance mammary expression as expected. Instead, this multimerization of the promoter region reduced expression in both cell cultures and transgenic mice.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Mammalian Genetics

Background:

  • Transcription factor binding sites are crucial for gene expression.
  • Locus control regions (LCRs) enhance gene expression in specific tissues.
  • The beta-lactoglobulin (BLG) gene is a key marker for mammary gland expression.

Purpose of the Study:

  • To create a synthetic LCR for mammary-specific gene expression.
  • To investigate the effect of multiple transcription factor binding sites on BLG gene expression.

Main Methods:

  • Generated reporter constructs with multimerized BLG promoter regions.
  • Tested constructs via stable transfection in mammary epithelial cells (in vitro).
  • Evaluated constructs in transgenic mice (in vivo).

Related Experiment Videos

  • Assessed DNaseI hypersensitive site formation in mammary chromatin.

    • Main Results:

    • Multimerization of BLG promoter regions did not enhance expression.
    • Expression was reduced in both in vitro and in vivo models.
    • The multimerized region failed to form an autonomous DNaseI hypersensitive site.

    Conclusions:

    • Not all combinations of transcription factor binding sites enhance transcription.
    • Synthetic LCR design requires careful consideration of site arrangement and chromatin interactions.
    • The BLG promoter multimerization strategy was unsuccessful in creating a functional LCR.