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Ultrastructural and immunohistochemical findings in five patients with vitreomacular traction syndrome
K Shinoda1, A Hirakata, T Hida
1Department of Ophthalmology, Kyorin University School of Medicine, Mitaka-shi, Japan.
Objective:
To evaluate pathologic features of vitreomacular traction syndrome (VMT).
Methods:
Preretinal membranes removed from five patients during vitreous surgery for VMT syndrome were evaluated by electron microscopy (n = 4) and immunohistochemistry (n = 1).
Results:
Electron microscopic examination revealed large segments of internal limiting lamina (ILL) in three of the four cases. Other extracellular features included two types of abnormal collagen fibrils, determined to be type I and fibrous long-spacing collagen. The myofibrocyte was the predominant cell type in all cases. Collagen types I, II, and III, as well as glial fibrillary acidic protein and vimentin, were identified by immunohistochemistry.
Conclusions:
Epiretinal membranes in eyes with VMT syndrome adhered tightly to the ILL with abnormal collagen and contractile elements that included myofibrocytes.
Insights
Vitreomacular traction syndrome (VMT) involves epiretinal membranes strongly attached to the internal limiting lamina (ILL). These membranes contain abnormal collagen and contractile myofibrocytes, impacting vision.
Area of Science:
- Ophthalmology
- Retinal Diseases
- Pathology
Background:
- Vitreomacular traction syndrome (VMT) is a condition affecting the macula.
- Understanding the pathological features of epiretinal membranes in VMT is crucial for treatment.
Purpose of the Study:
- To investigate the detailed pathologic features of epiretinal membranes in patients with VMT syndrome.
Main Methods:
- Preretinal membranes from five VMT patients were analyzed.
- Techniques included electron microscopy (n=4) and immunohistochemistry (n=1).
Main Results:
- Internal limiting lamina (ILL) fragments were present in most samples.
- Abnormal collagen types (I and fibrous long-spacing) and myofibrocytes were identified.
- Immunohistochemistry confirmed collagen types I, II, III, GFAP, and vimentin.
Conclusions:
- Epiretinal membranes in VMT syndrome exhibit tight adhesion to the ILL.
- Abnormal collagen and contractile myofibrocytes are key components of these membranes.