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IS1294, a DNA element that transposes by RC transposition.

N Tavakoli1, A Comanducci, H M Dodd

  • 1Laboratoire de Microbiologie et de Génétique, UPRES A 7010-CNRS, 28, rue Goethe, Strasbourg Cedex, 67083, France.

Plasmid
|June 30, 2000
PubMed
Summary

IS1294, an active insertion sequence, moves using rolling-circle replication. This process, initiated at oriIS and terminated at terIS, allows IS1294 to transpose itself and adjacent DNA sequences.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • IS1294 is an active 1.7-kb insertion sequence found on the ColD-like resistance plasmid pUB2380.
  • It exhibits characteristics of IS91-like elements, including lack of terminal inverted repeats and insertion-site specificity.

Purpose of the Study:

  • To characterize the transposition mechanism of IS1294.
  • To elucidate the roles of oriIS and terIS in IS1294 transposition.
  • To present a model for the transposition of IS1294 and related elements.

Main Methods:

  • Sequence analysis of IS1294 and its flanking regions.
  • Identification and characterization of the transposase gene (tnp1294).
  • Analysis of oriIS and terIS sequences and their potential functions.

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Main Results:

  • IS1294 possesses a single open reading frame, tnp(1294), encoding a transposase related to REP family replication proteins.
  • Transposition occurs via rolling-circle replication, initiated at oriIS and terminated at terIS.
  • oriIS and terIS are conserved and resemble RC-plasmid replication origins and transcription terminators, respectively.
  • IS1294 mediates the transposition of adjacent DNA sequences.

Conclusions:

  • IS1294 utilizes a novel rolling-circle replication mechanism for transposition.
  • The conserved oriIS and terIS elements play crucial roles in the transposition process.
  • IS1294 can act as a mobile genetic element, facilitating the transfer of adjacent genetic material.