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IS1294, a DNA element that transposes by RC transposition
N Tavakoli1, A Comanducci, H M Dodd
1Laboratoire de Microbiologie et de Génétique, UPRES A 7010-CNRS, 28, rue Goethe, Strasbourg Cedex, 67083, France.
Plasmid
|June 30, 2000
Summary
IS1294, an active insertion sequence, moves using rolling-circle replication. This process, initiated at oriIS and terminated at terIS, allows IS1294 to transpose itself and adjacent DNA sequences.
Area of Science:
- Molecular Biology
- Genetics
- Microbiology
Background:
- IS1294 is an active 1.7-kb insertion sequence found on the ColD-like resistance plasmid pUB2380.
- It exhibits characteristics of IS91-like elements, including lack of terminal inverted repeats and insertion-site specificity.
Purpose of the Study:
- To characterize the transposition mechanism of IS1294.
- To elucidate the roles of oriIS and terIS in IS1294 transposition.
- To present a model for the transposition of IS1294 and related elements.
Main Methods:
- Sequence analysis of IS1294 and its flanking regions.
- Identification and characterization of the transposase gene (tnp1294).
- Analysis of oriIS and terIS sequences and their potential functions.
Main Results:
- IS1294 possesses a single open reading frame, tnp(1294), encoding a transposase related to REP family replication proteins.
- Transposition occurs via rolling-circle replication, initiated at oriIS and terminated at terIS.
- oriIS and terIS are conserved and resemble RC-plasmid replication origins and transcription terminators, respectively.
- IS1294 mediates the transposition of adjacent DNA sequences.
Conclusions:
- IS1294 utilizes a novel rolling-circle replication mechanism for transposition.
- The conserved oriIS and terIS elements play crucial roles in the transposition process.
- IS1294 can act as a mobile genetic element, facilitating the transfer of adjacent genetic material.