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Molecular cloning of a cyclin-like protein associated with cyclin-dependent kinase 3 (cdk 3) in vivo

M Matsuoka1, Y Matsuura, K Semba

  • 1Department of Pharmacology, Keio University School of Medicine, Shinanomachi 35, Shinjuku-ku, Tokyo, 160-8582, Japan. sakimatu@mc.med.keio.ac.jp

Biochemical and Biophysical Research Communications
|June 30, 2000
PubMed

Insights

Researchers identified a novel cyclin-like protein, ik3-1, that interacts with cyclin-dependent kinase 3 (cdk3). This interaction suggests a role for ik3-1 in regulating mammalian cell cycle progression during the G1 phase.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cyclin-dependent kinase 3 (cdk3) is implicated as a rate-limiting factor in mammalian cell cycle progression.
  • The precise function of cdk3 and its regulatory partners remain incompletely understood.

Purpose of the Study:

  • To elucidate the function of cdk3 by identifying its interacting proteins.
  • To characterize a novel cyclin-like protein interacting with cdk3.

Main Methods:

  • Yeast two-hybrid system was employed to screen for cdk3 interactors.
  • Isolation and characterization of a cyclin-like cDNA, designated ik3-1.
  • Coimmunoprecipitation assays to confirm in vivo interaction between p70(ik3-1) and p35(cdk3).

Main Results:

  • A novel cyclin-like protein, p70(ik3-1), was identified as an interactor of cdk3.
  • The C-terminal domain of p70(ik3-1) exhibits homology to the conserved cyclin box.
  • p70(ik3-1) was shown to bind to p35(cdk3) in vivo.
  • ik3-1 gene expression is upregulated during the G1 phase of the cell cycle.

Conclusions:

  • The identified protein, p70(ik3-1), is a novel cyclin that interacts with cdk3.
  • The temporal expression pattern of ik3-1 suggests its primary role in regulating the G1 phase of the cell cycle.
  • This discovery provides new insights into the molecular mechanisms governing cell cycle progression.

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